区别就是两者的人称代词宾语不一样，具体的不同如下
wake him up中文意思是把他叫醒
She gave him a pinch on the arm to wake him up.
她拧一下他的胳膊把他唤醒。
wake her up中文意思是把她叫醒
She is asleep but I will wake her up.她睡着了，可是我要把她弄醒。
wake him up和wake her up
wake him up 和wake her up 的区别仅仅在于作宾语的代词不同：him 是男性，而her 是女性，它们的中文意思分别是：“把他叫醒” 和 “把她叫醒”。
wake up是个短语动词，它可以是及物的，也可以是不及物的。
作及物动词用时，意思是：把某人叫醒，如果宾语是名词，既可以放在make和up之间，也可以放在up之后如果宾语是代词，则只能放在make和up之间。例如：Every morning the mother has to wake up her daughter. / His daughter wants to sleep for longer time. so he won't wake her up until 7:00 o'click.
作不及物动词用时，意思是：醒来。例如：He usually wakes up at 6:30 in the morning.
回答完毕。
wake him up和wake her up
wakes him up 叫醒他
wakes her up 叫醒她
wake sb. up 叫醒某人，唤醒某人
him 是 he 的宾格，意思是 ”他“。
her 是 she 的宾格，意思是”她“。
以上是所有回答，希望帮到您。
